Summary
Type: TLC
Application #: 400420
Catalog#: 3522026 - Polyamide TLC Plates, polyester backed, 100um, 20x20cm 25/pk
Detection: 1. spray with 10% aqueous phosphoric acid, 2. heat 10 min to 120 °C, 3. spray hot plates with 10% phosphormolybdic acid and heat at 120 °C to maximum colouration (about 6 - 8 min) (see chapter visuali
Conditions: Eluent:
A) water / butanone-(2) / 25% ammonia (80:20:5, v/v)
B) 0.05 N sodium hydroxide solution
Authors: Freimuth U
Source: J Chromatogr 30 (1967) 607
Details
Legend: Substance
Cholanic acid OH position
hRf in eluent A
hRf in eluent B
Lithocholic acid
3 a
21
2
Hyodeoxycholic acid
3 a, 6 a
39
9
Chenodeoxycholic acid
3 a, 7 a
53
16
Deoxycholic acid
3